mouse mip 3α  (Elabscience Biotechnology)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Unraveling CCL20's role by regulating Th17 cell chemotaxis in experimental autoimmune prostatitis

doi: 10.1111/jcmm.18445

Figure Lengend Snippet: The increased levels of CCL20 in the prostate in experimental autoimmune prostatitis mice. (A) Immunohistochemical staining of CCL20 on paraffin‐embedded prostate sections. (B) Semi‐quantitative analysis of CCL20 in Immunohistochemical staining. (C) Relative expression of CCL20 and CCR6 in the prostate measured by RT‐qPCR. (D–F) The expression level of CCL20 and CCR6 in the prostate tissues assessed by Western Blot. (G, H) Quantitative analysis of CCL20 in the serum and prostate of mice determined by ELISA. Representative data from three independent experiments are shown. Data were presented as mean ± SD and were analysed using unpaired, two‐tailed Student's t‐test analysis. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The cytokines levels in serum of mice were measured by employing the Elisa kit for IL‐17A (Elabscience, E‐EL‐M0047c), IL‐17F (Multi‐Science, EK2196), IL‐22 (Elabscience, E‐EL‐M2446), IFN‐γ (Elabscience, E‐EL‐M0048), TNF‐α (Elabscience, E‐EL‐M3063), GM‐CSF (Elabscience, E‐MSEL‐M0022) and CCL20 (Elabscience, E‐EL‐M0013).

Techniques: Immunohistochemical staining, Staining, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Journal of Cellular and Molecular Medicine

Article Title: Unraveling CCL20's role by regulating Th17 cell chemotaxis in experimental autoimmune prostatitis

doi: 10.1111/jcmm.18445

Figure Lengend Snippet: The recruitment of Th17 cells by CCL20. (A, D) Counting Th17 cells chemoattracted by CCL20 through transwell assay. (B) Immunohistochemical staining of IL‐17A on paraffin‐embedded prostate sections in each group. (C) Assessment of cell viability of Th17 cells after treatment with rCCL20 or anti‐CCL20 neutralizing antibodies. (E) Semi‐quantitative analysis of CCL20 in Immunohistochemical staining. (F) Quantitative analysis of IL‐17A in the serum of mice determined by ELISA. (G, H) Flow cytometry analysis of CD4 + IL‐17A + cells in the prostate. (I–K) The expression level of IL‐17A and CCR6 in the prostate tissues determined by Western Blot. (L) Relative expression of IL‐17A and CCR6 in the prostate measured by RT‐qPCR. Representative data from three independent experiments are shown. (M, N) Quantitative analysis of inflammatory cytokines (IL‐17A, IL‐17F, IL‐22, IFN‐γ, TNF‐α, GM‐CSF) in the serum and prostate of mice determined by ELISA. Data were presented as mean ± SD and were analysed using one‐way anova analysis. ‘N.D.’ Not Determined; ‘ns’ p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The cytokines levels in serum of mice were measured by employing the Elisa kit for IL‐17A (Elabscience, E‐EL‐M0047c), IL‐17F (Multi‐Science, EK2196), IL‐22 (Elabscience, E‐EL‐M2446), IFN‐γ (Elabscience, E‐EL‐M0048), TNF‐α (Elabscience, E‐EL‐M3063), GM‐CSF (Elabscience, E‐MSEL‐M0022) and CCL20 (Elabscience, E‐EL‐M0013).

Techniques: Transwell Assay, Immunohistochemical staining, Staining, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Expressing, Western Blot, Quantitative RT-PCR

Journal: Journal of Cellular and Molecular Medicine

Article Title: Unraveling CCL20's role by regulating Th17 cell chemotaxis in experimental autoimmune prostatitis

doi: 10.1111/jcmm.18445

Figure Lengend Snippet: The potential source CCL20 in the prostate of experimental autoimmune prostatitis (EAP) mice. Representative photographs of immunofluorescence staining for CCL20 and markers for (A) CD4 + T cells, (B) macrophages and (C, D) prostatic stromal cells of EAP mice. (E) Quantification of CCL20 immunofluorescence intensity in the experiments of (A–D). Representative data from three independent experiments are shown. Data were presented as mean ± SD and were analysed using one‐way anova analysis. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The cytokines levels in serum of mice were measured by employing the Elisa kit for IL‐17A (Elabscience, E‐EL‐M0047c), IL‐17F (Multi‐Science, EK2196), IL‐22 (Elabscience, E‐EL‐M2446), IFN‐γ (Elabscience, E‐EL‐M0048), TNF‐α (Elabscience, E‐EL‐M3063), GM‐CSF (Elabscience, E‐MSEL‐M0022) and CCL20 (Elabscience, E‐EL‐M0013).

Techniques: Immunofluorescence, Staining

Journal: Journal of Cellular and Molecular Medicine

Article Title: Unraveling CCL20's role by regulating Th17 cell chemotaxis in experimental autoimmune prostatitis

doi: 10.1111/jcmm.18445

Figure Lengend Snippet: IL‐17A promoted secretion of CCL20 of macrophages via the NF‐κB/MAPK/PI3K pathway. (A, B) Western blot analysis of CCL20 and the phosphorylation of the PI3K (mTOR and AKT) NF‐κB (P65) and MAPK (ERK1/2, P38) pathways in BMDM and RAW264.7 after treatment of LPS and/or rIL‐17. (C, D) Quantitative analysis of CCL20 in the supernatant of BMDM and RAW264.7 determined by ELISA. (E, F) Relative expression of CCL20 in BMDM and RAW264.7 measured by RT‐qPCR after treatment of LPS and/or rIL‐17A. (G, H) Western blot analysis of CCL20 after injection of rIL‐17A or anti‐IL‐17A neutralizing antibodies. (I, J) Quantitative analysis of CCL20 in the serum and prostate of mice determined by ELISA. Representative data from three independent experiments are shown. Data were presented as mean ± SD and were analysed using one‐way anova analysis. ‘ns’ p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The cytokines levels in serum of mice were measured by employing the Elisa kit for IL‐17A (Elabscience, E‐EL‐M0047c), IL‐17F (Multi‐Science, EK2196), IL‐22 (Elabscience, E‐EL‐M2446), IFN‐γ (Elabscience, E‐EL‐M0048), TNF‐α (Elabscience, E‐EL‐M3063), GM‐CSF (Elabscience, E‐MSEL‐M0022) and CCL20 (Elabscience, E‐EL‐M0013).

Techniques: Western Blot, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Injection